This WP aims to characterise the active substances with biocidal activity from extracts obtained from different raw materials, and optimise and validate the extraction processes needed for their isolation, based on a pharmacological approach. The result of this WP would be the comprehensive characterisation of the target active substances with insecticidal, nematocidal and fungicidal properties, as well as the optimisation of the exhaustively controlled extraction processes for the later scaling up. The results of the characterisation will be used as an essential part of the dossier needed for the active substances registration at a further stage.